Translation

1) What are homopolymers use in discovering the genetic code? How were they made and used?

2) How can you use heteropolymers with two precursur bases in specific ratios to resolve the genetic code?

3) What considerations led scientists to hypothesize that the code was a triplet, non-overlapping code?

4) Consider this gene. Show the m-RNA synthesized from the sense strand.

            3'_________________________________5'....... __________
                |    |    |    |    |    |    |    |    |    |    |    |    |    |            |                    |
                A  A  T  G  C  C  C  A   T  T  G  G  C   A           |                    |
                                                                                          PROMOTER
                T  T   A  C  G  G  G  T   A  A C  C  G   T           |                    |
           5'   |    |    |    |    |    |    |    |    |    |    |    |    |    | 3' ..... |                    |

5)Consider the eukaryotic DNA sense strand below (in colors). Construct the hn-RNA(initial m-RNA transcript) , m-RNA, and Polypeptide encoded by this DNA. You will have access to a genetic code on an exam - use your book or the web for this one.

promoter

1st base transcribed

Exons

Intron

terminator

3' - TATACCCTATCCGTACGCATCCGCGCGATAAAACTGGGCCGCATCCGGATGGCCT - 5'

hn-RNA:

m-RNA:

Polypeptide: